Targets - OLD stuff

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OLD STUFF
Sadhana

m
Protocol Target Discovery
ORGANISM: MYCOBACTERIUM TUBERCULOSIS H37Rv
EC# 3.1.3.16
TARGET ENZYME: protein-tyrosine-phosphatase
GENE OF INTEREST: ppp serine/threonine phosphatase
NCBI GENE # OR REFSEQ #:887070
CDS GENE SEQUENCE:
>gi|57116681:c23181-21637 Mycobacterium tuberculosis H37Rv, complete genome
GTGGCGCGCGTGACCCTGGTCCTGCGATACGCGGCGCGCAGCGATCGCGGCTTGGTACGCGCCAACAACGAAGACTCGGTCTACGCTGGGGCACGGCTATTGGCCCTGGCCGACGGCATGGGTGGGCATGCGGCCGGCGAGGTGGCGTCCCAGTTGGTGATTGCCGCATTGGCCCATCTCGATGACGACGAGCCCGGTGGCGATCTGCTGGCCAAGCTGGATGCCGCGGTGCGCGCCGGCAACTCGGCTATCGCAGCGCAAGTCGAGATGGAGCCCGATCTCGAAGGCATGGGTACCACGCTCACCGCAATCCTGTTCGCGGGCAACCGGCTCGGCCTGGTGCATATCGGTGACTCGCGCGGTTACCTGCTGCGCGACGGTGAGCTGACGCAGATCACCAAGGACGACACGTTTGTCCAA
ACGCTGGTCGACGAAGGCCGGATCACCCCGGAGGAGGCGCACAGCCACCCGCAACGCTCGTTGATCATGCGGGCGTTGACCGGCCATGAGGTCGAACCGACGCTGACCATGCGAGAAGCCCGCGCCGGTGATCGTTACCTGCTGTGCTCGGACGGGTTGTCCGATCCGGTTAGCGATGAAACTATCCTCGAGGCCCTGCAGATCCCCGAGGTTGCCGAGAGCGCTCACCGCCTCATTGAACTGGCGCTGCGCGGCGGCGGCCCCGACAACGTCACTGTCGTCGTCGCCGACGTCGTCGACTACGACTACGGCCAGACCCAACCGATTCTGGCCGGGGCGGTCTCAGGCGACGACGACCAACTGACCCTGCCCAACACCGCCGCCGGCCGGGCCTCTGCCATCAGCCAGCGCAAGGAGATCGTTAAACGCGTTCCGCCACAGGCCGATACATTCAGTCGGCCACGGTGGTCGGGCCGACGGCTAGCATTCGTTGTCGCACTGGTGACCGTGCTGATGACTGCGGGCCTGCTCATTGGTCGCGCGATCATCCGCAGCAACTACTACGTAGCGGACTACGCCGGCAGCGTGTCCATCATGCGGGGGATTCAAGGGTCGCTACTGGGCATGTCCCTGCACCAGCCTTACCTGATGGGCTGCCTCAGCCCGCGTAACGAGCTGTCGCAGATCAGCTACGGACAGTCTGGGGGCCCTCTCGACTGCCATCTGATGAAACTGGAGGATCTGCGACCGCCGGAGCGCGCACAGGTTCGGGCCGGTCTCCCGGCCGGCACTCTCGATGACGCCATCGGGCAGTTGCGCGAACTGGCGGCCAACTCCCTG
CTGCCGCCTTGCCCGGCGCCGCGTGCCACGTCCCCGCCCGGGCGCCCGGCCCCACCCACCACCAGCGAGACAACCGAACCAAACGTCACCTCCTCGCCAGCCTCTCCATCACCCACCACCTCCGCGCCGGCCCCCACCGGAACTACTCCTGCCATCCCCACGAGTGCCTCCCCGGCAGCGCCCGCGTCGCCGCCGACGCCTTGGCCCGTCACCAGCTCGCCGACGATGGCCGCACTTCCGCCACCCCCGCCTCAGCCGGGCATCGACTGCCGGGCGGCGGCATGA

AMINO ACID SEQUENCE:
>1TXO:A|PDBID|CHAIN|SEQUENCE

MARVTLVLRYAARSDRGLVRANNEDSVYAGARLLALADGMGGHAAGEVASQLVIAALAHLDDDEPGGDLLAKLDAAVRAGNSAIAAQVEMEPDLEGMGTTLTAILFAGNRLGLVHIGDSRGYLLRDGELTQITKDDTFVQTLVDEGRITPEEAHSHPQRSLIMRALTGHEVEPTLTMREARAGDRYLLCSDGLSDPVSDETILEALQIPEVAESAHRLIELALRGGGPDNVTVVVADVVD


PDB STRUCTURE IDENTIFIER: 2CM1
Joshua

ORGANISM: Mycobacterium tuberculosis H37Rv
EC# of Enzyme: 3.1.3.16
GENE of INTEREST: ppp serine/threonine phosphatase
NCBI GENE #: 887070
CDS GENE SEQUENCE:
>gi|57116681:c23181-21637 Mycobacterium tuberculosis H37Rv, complete genome
GTGGCGCGCGTGACCCTGGTCCTGCGATACGCGGCGCGCAGCGATCGCGGCTTGGTACGCGCCAACAACGAAGACTCGGTCTACGCTGGGGCACGGCTATTGGCCCTGGCCGACGGCATGGGTGGGCATGCGGCCGGCGAGGTGGCGTCCCAGTTGGTGATTGCCGCATTGGCCCATCTCGATGACGACGAGCCCGGTGGCGATCTGCTGGCCAAGCTGGATGCCGCGGTGCGCGCCGGCAACTCGGCTATCGCAGCGCAAGTCGAGATGGAGCCCGATCTCGAAGGCATGGGTACCACGCTCACCGCAATCCTGTTCGCGGGCAACCGGCTCGGCCTGGTGCATATCGGTGACTCGCGCGGTTACCTGCTGCGCGACGGTGAGCTGACGCAGATCACCAAGGACGACACGTTTGTCCAA
ACGCTGGTCGACGAAGGCCGGATCACCCCGGAGGAGGCGCACAGCCACCCGCAACGCTCGTTGATCATGCGGGCGTTGACCGGCCATGAGGTCGAACCGACGCTGACCATGCGAGAAGCCCGCGCCGGTGATCGTTACCTGCTGTGCTCGGACGGGTTGTCCGATCCGGTTAGCGATGAAACTATCCTCGAGGCCCTGCAGATCCCCGAGGTTGCCGAGAGCGCTCACCGCCTCATTGAACTGGCGCTGCGCGGCGGCGGCCCCGACAACGTCACTGTCGTCGTCGCCGACGTCGTCGACTACGACTACGGCCAGACCCAACCGATTCTGGCCGGGGCGGTCTCAGGCGACGACGACCAACTGACCCTGCCCAACACCGCCGCCGGCCGGGCCTCTGCCATCAGCCAGCGCAAGGAGATCGTTAAACGCGTTCCGCCACAGGCCGATACATTCAGTCGGCCACGGTGGTCGGGCCGACGGCTAGCATTCGTTGTCGCACTGGTGACCGTGCTGATGACTGCGGGCCTGCTCATTGGTCGCGCGATCATCCGCAGCAACTACTACGTAGCGGACTACGCCGGCAGCGTGTCCATCATGCGGGGGATTCAAGGGTCGCTACTGGGCATGTCCCTGCACCAGCCTTACCTGATGGGCTGCCTCAGCCCGCGTAACGAGCTGTCGCAGATCAGCTACGGACAGTCTGGGGGCCCTCTCGACTGCCATCTGATGAAACTGGAGGATCTGCGACCGCCGGAGCGCGCACAGGTTCGGGCCGGTCTCCCGGCCGGCACTCTCGATGACGCCATCGGGCAGTTGCGCGAACTGGCGGCCAACTCCCTG
CTGCCGCCTTGCCCGGCGCCGCGTGCCACGTCCCCGCCCGGGCGCCCGGCCCCACCCACCACCAGCGAGACAACCGAACCAAACGTCACCTCCTCGCCAGCCTCTCCATCACCCACCACCTCCGCGCCGGCCCCCACCGGAACTACTCCTGCCATCCCCACGAGTGCCTCCCCGGCAGCGCCCGCGTCGCCGCCGACGCCTTGGCCCGTCACCAGCTCGCCGACGATGGCCGCACTTCCGCCACCCCCGCCTCAGCCGGGCATCGACTGCCGGGCGGCGGCATGA

AMINO ACID SEQUENCE:
>2CM1:A|PDBID|CHAIN|SEQUENCE
MGSSHHHHHHSSGLVPRGSHMARVTLVLRYAARSDRGLVRANNEDSVYAGARLLALADGMGGHAAGEVASQLVIAALAHLDDDEPGGDLLAKLDAAVRAGNSAIAAQVEMEPDLEGMGTTLTAILFAGNRLGLVHIGDSRGYLLRDGELTQITKDDTFVQTLVDEGRITPEEAHSHPQRSLIMRALTGHEVEPTLTMREARAGDRYLLCSDGLSDPVSDETILEALQIPEVAESAHRLIELALRGGGPDNVTVVVADVVD

PDB Structure Identifier: 2CM1
Hong
ORGANISM: GENE of INTEREST:
NCBI GENE # or RefSeq#: EC# of Enzyme:
CDS GENE SEQUENCE:
AMINO ACID SEQUENCE:
Jeanette
ORGANISM: Trypanosoma brucei
GENE of INTEREST: acid phosphatase (Not enough similarity to a PDB structure)
NCBI GENE # or RefSeq#: 3661968
EC# of Enzyme: 3.1.3.2
CDS GENE SEQUENCE:
>gi|73747658:1747242-1748549 Trypanosoma brucei TREU927 chromosome 10, complete genome

ATGTTCCTTTTGCCAAGGGAAGTAATGAGGAAGTACGCCATATTTTCCTTAGCAATCATACTGTTGCTAT

TTACAGTAGTGAGCAGTGAGCCTGGTGAGCGCGATGCGGAGTCCGGGGTGAAGAGAGGGGACGAAAAGCA

GAACCTCGTCCTCACTAAGCTAATTGTTCTCAACCGTCATGGTCACCGCGCCCCTAACGCCCCCTACTGG

TCCATGTGCCCCAACGACAGAAAAAACCGCCGCAAGTACAACGTTGGCGCGGAGGACCTTACGGCGCGCG

GCATGAAGGAGGAGTATGAATTGGGGGAGTATTTACGGAACGCGTATCGCGACTTTATTGGACCTCGCTT

CAACCGAAGTCGGCACTTCCTGCGCGCCGTGGGCGAACCCCGAATTCTGCAGTCCGCTCAGGCGGTTGCC

CAAGGGCTCTTTCCTGATGGTTATGGGCCCGGTGGCTATCTTCCGCGACAACCTCAGTTTGTGCCCGTAT

TTTCTGATATGGATACTCATGAATATCTTCTCGACGACGTACCGTGCTTCCGCCGCGCTGAGAGGGATAT

GCGCAGATGGTTGAACACTAGCTTGAGTGACTTCATTGCTGACCCTAAAGTCAGCGAGGTGTTAAACTAT

ATGAAACGGATGTGTGGGGTGTCTGAGAAGGACATGCCACCCGTATATGCTTTTCTGAAAACTGTAGCCG

ACGGGATGACATTCAATGCCGATTTCGGACTGAGCGTGTGCCATGGGAAGGTAACACCCGAAATGCTCTT

CAAAATACGCAAAGTATCACTACGACTTCTTATGGCGCGACTTTATCACACTGACGAGCAACAAACCTAC

ACCTCTGTAGATTTACCATACCGGATACTCTATATGCTAAATCGGTCAACCTCCCCAGGTGCGAAGGGAG

TGAACGATTACTCCGACACAGAGCAGGAAGCCATCCTGTATTTTATGCATCGCGAGGCCCTGTACGCTGT

TGGAGAGTTCTTTGGTTTCACATTTAATGTCCCAGGTCTGCCGCACGGGGAGCTGCCAGTGGCTTCTACA

ATTGTATGGGAGCGGTTGGTTCCTGAGAAGAGCGTCGGGGAAGAGATTGTGCCATCAAGAACGTACGTGC

GTCTCATACTATGGACACCAAAGGATGGTACAGCCACGGTTGCCATACCGCACTGCAGAACACCCCAGCT

TTGCACACTCAAAGAACTTCAGGATATATACAGTGCGCGGGTGAAGCGCACTGGATCGTGGTTGAAGCTG

TGCAACTTCACAATCACAGAATTAGACCATGACACGGACATTCGTTAG
AMINO ACID SEQUENCE:
Marshall
ORGANISM: GENE of INTEREST:
NCBI GENE # or RefSeq#: EC# of Enzyme:
CDS GENE SEQUENCE:
AMINO ACID SEQUENCE:

Bubonic Plague
caused by bacteria Yersinia Pestis
EC 3.6.1.11
Structure of Exopolyphosphatase from Yersinia pestis
<http://www.pdb.org/pdb/explore/explore.do?structureId=3RF0>

African Sleeping Sickness
caused by protozoan Trypanosoma brucei
pdb number 3M4U
EC 3.1.3.48
Enzyme is Protein-Tyrosine-Phosphatase
<http://www.pdb.org/pdb/explore/explore.do?structureId=3M4U>
Justin
ORGANISM:Toxoplasma gondii ME49 GENE of INTEREST: TGME49_031850 (serine-threonine phosophatase 2C)


NCBI GENE # or RefSeq#: 7898899 EC# of Enzyme:3.1.3.16

CDS GENE SEQUENCE:
 1 atgaagtcct ctgctgaaat taggcggacc atggatgtcc ctcctaccat tcatgtacct
 61 ctccctccaa cgtcgtatcc cgctttcgat gctgcgatct tcacagacat cggtgggcgc
 121 aagcatcagg aagataggtt cactctctgt ccgcagctcg ttcccggccg agacgactgc
 181 gccttcttcg gtgtcttcga tggcactgtt ggagatttcg ccagcgaaaa tgtgaaggat
 241 cttgttgttc cacagttgat ttcctcgccc gcgtggcagg aggtgactga gatgctgaga
 301 tcagacgtac ccgccactga ggtggacgag aagctccctc agttgcttga tcaggcagtc
 361 gatgacatgt acaagaacgc agacaacgaa cttgtgaaga tgtgcgagca gcttaacaaa
 421 gactacgcga gcagcacctc cgtcacggca gtcttggcca aaggcttcgt ggctgttggt
 481 catctgggcg acagccgcat cgctatggga gtcgagacgc caaacgggtt gaactgcgag
 541 ttcttgaccg ttgaccacaa gccggatatg ccacatgaga aactgcgcat catgcgcaat
 601 ggaggcagtg ttgagtatct ccacaaccac aacaacaaac cgttcattcg aggtggtgac
 661 ttctcgttcc ggaagtcgcg cggagagcag ccgatgcagc tccagtactc ccgagctttc
 721 ggcgggaagg acctgaagat gtacggtttg agcaaccaac ccgacgtacg cgttgtccgc
 781 gtcacgcctc aacaccgagt gatgattctc gcgactgatg gcttgtggga cgtcatgtct
 841 gcggcgcaag ctgtagagat cgctatgcag gcacgacagg aaggaaggaa cccagcgcag
 901 gcgctggtgg agatgaccct cgctgagcag cagagccgca accaaagtgc agacaacatt
961 actgcgatga cagtgttctt caagaagact gattag
AMINO ACID SEQUENCE: (Justin - is this the truncated sequence?? - Dr. B)
 
[[code]]
<span style="font-family: 'Courier New';">SLTMDVPPTIHVPLPPTSYPAFDAAIFTDIGGRKHQEDRFTLCPQLVPGRDDCAFFGVFDGTVGDFASENVKD</span>
[[code format="genbank"]]
 
LVVPQLISSPAWQEVTEMLRSDVPATEVDEKLPQLLDQAVDDMYKNADNELVKMCEQLNKDYASSTSVTAVLA
 
[[code]]
<span style="font-family: 'Courier New';">KGFVAVGHLGDSRIAMGVETPNGLNCEFLTVDHKPDMPHEKLRIMRNGGSVEYLHNHNNKPFIRGGDFSFRKS</span>
[[code format="genbank"]]
 
RGEQPMQLQYSRAFGGKDLKMYGLSNQPDVRVVRVTPQHRVMILATDGLWDVMSAAQAVEIAMQARQEGRNPA
 
[[code]]
<span style="font-family: 'Courier New';">QALVEMTLAEQQSRNQSADNTAMTVFFKKTD</span>
[[code format="genbank"]]
 
PDB
2I44_A
Larry
ORGANISM:
Yersinia enterocolitica
GENE of INTEREST:
phosphoanhydride phosphorylase
 
[[code]]
<span style="font-size: 14px;">NCBI GENE # or RefSeq#:</span>
<span style="background-color: transparent; color: #000000; font-family: Arial; font-size: 14px; text-decoration: none; vertical-align: baseline;">4714245</span>
<span style="font-size: 14px;">EC# of Enzyme: 3.1.3.48</span>
<span style="font-size: 14px;">CDS GENE SEQUENCE:</span>
=<span style="background-color: transparent; color: #000000; font-family: Arial; font-size: 14px; text-decoration: none; vertical-align: baseline;">ATGTCAGTTGCAAAGAGAAATCTGCACTTATCCGCACTCACTTTGATATTGGGCTGTTTTACCGCAGGTG</span>= 
[[code format="genbank"]]
 

CTGCCCCGATTGCTACACCGCCGGCCGGCTACACATTAGAGCGTGTGGTTATTTTGAGTCGACATGGTGT

 
[[code]]
=<span style="background-color: transparent; color: #000000; font-family: Arial; font-size: 14px; text-decoration: none; vertical-align: baseline;">TCGCTCCCCGACAAAACAAACCCAGCTAATGAATGATGTCACACCTGATAAATGGCCCCTGTGGCCAGTA</span>= 
[[code format="genbank"]]
 

AAAGCGGGCTATTTAACACCGCGAGGGGCCGAGTTAGTGACTTTGATGGGGGGATTTTATGGTGATTATT

 
[[code]]
=<span style="background-color: transparent; color: #000000; font-family: Arial; font-size: 14px; text-decoration: none; vertical-align: baseline;">TCCGCAGCCAAGGGTTGTTGTCTGCGGGGTGTCCGGTAGATGGCTCCGTTTATGCACAGGCAGATGTTGA</span>= 
[[code format="genbank"]]
 

CCAACGAACCCGCTTAACCGGACAGGCATTCTTGGATGGGATCGCACCGGATTGTGGTCTGAAAGTACAT

 
[[code]]
=<span style="background-color: transparent; color: #000000; font-family: Arial; font-size: 14px; text-decoration: none; vertical-align: baseline;">TATCAGGCTGATTTGAAGAAAGTTGACCCGCTATTTCATACCGTCGAAGCGGGGGTCTGTAAACTGGACT</span>= 
[[code format="genbank"]]
 

CAGCGAAAACTCATCAGGCTGTTGAGGAGCGATTGGGCGGGCCATTGAGTGATCTTAGCCAGCGCTATGC

 
[[code]]
=<span style="background-color: transparent; color: #000000; font-family: Arial; font-size: 14px; text-decoration: none; vertical-align: baseline;">CAAACCCTTTGCTCAGATGGGCGAAGTGCTGAATTTTGCAGCATCGCCTTATTGCAAGTCATTGCAAAAA</span>= 
[[code format="genbank"]]
 

AATGGAAAAACCTGTGATTTTGCAACTTTTACGGCAAATGAAATTAAGGTAAACGAAGAAGGTACTAAAG

 
[[code]]
=<span style="background-color: transparent; color: #000000; font-family: Arial; font-size: 14px; text-decoration: none; vertical-align: baseline;">TTTCTCTGAGTGGGCCATTGGCACTATCGTCGACATTGGGTGAAATTTTCCTGTTACAAAACTCACAAGC</span>= 
[[code format="genbank"]]
 

TATGCCGGATGTGGCCTGGCATCGGCTCAGCGGTGAAGAGAACTGGGTTTCGCTATTGTCGTTGCACAAT

 
[[code]]
=<span style="background-color: transparent; color: #000000; font-family: Arial; font-size: 14px; text-decoration: none; vertical-align: baseline;">GCGCAATTTGATTTGATGGCCAAAACCCCGTATATCGCTCGCCATAAAGGGACCCCGCTGTTGCAACAAA</span>= 
[[code format="genbank"]]
 

TTGATACGGCATTAGTGCTGCAACGCGATGCCCAAGGGCAAACACTGCCGCTGTCACCGCAAACCAAATT

 
[[code]]
=<span style="background-color: transparent; color: #000000; font-family: Arial; font-size: 14px; text-decoration: none; vertical-align: baseline;">GCTGTTCCTCGGCGGGCATGACACCAATATTGCTAATATCGCTGGTATGTTAGGGGCCAATTGGCAATTA</span>= 
[[code format="genbank"]]
 

CCACAGCAACCTGATAATACCCCGCCTGGTGGCGGATTAGTCTTTGAGCTATGGCAGAACCCAGATAATC

 
[[code]]
=<span style="background-color: transparent; color: #000000; font-family: Arial; font-size: 14px; text-decoration: none; vertical-align: baseline;">ATCAGCGCTATGTCGCCGTGAAAATGTTCTATCAAACGATGGATCAGCTGCGAAATGCCGAGAAATTAGA</span>= 
[[code format="genbank"]]
 

TATGAAAAACAACCCAGCTAAAATTGTTCCAATTACCATTGAAGGTTGTGAGAACGAGGGTGATAACAAA

 
[[code]]
=<span style="background-color: transparent; color: #000000; font-family: Arial; font-size: 14px; text-decoration: none; vertical-align: baseline;">CTTTGCCAACTTGAGACTTTCCAAAAGAAAGTTGCCCAAGTGATAGAGCCAGCTTGCCATATTTAA</span>= 
[[code format="genbank"]]
 
AMINO ACID SEQUENCE:

MSVAKRNLHLSALTLILGCFTAGAAPIATPPAGYTLERVVILSRHGVRSPTKQTQLMNDV

 
[[code]]
=<span style="background-color: transparent; color: #000000; font-family: Arial; font-size: 14px; text-decoration: none; vertical-align: baseline;">TPDKWPLWPVKAGYLTPRGAELVTLMGGFYGDYFRSQGLLSAGCPVDGSVYAQADVDQRT</span>= 
[[code format="genbank"]]
 

RLTGQAFLDGIAPDCGLKVHYQADLKKVDPLFHTVEAGVCKLDSAKTHQAVEERLGGPLS

 
[[code]]
=<span style="background-color: transparent; color: #000000; font-family: Arial; font-size: 14px; text-decoration: none; vertical-align: baseline;">DLSQRYAKPFAQMGEVLNFAASPYCKSLQKNGKTCDFATFTANEIKVNEEGTKVSLSGPL</span>= 
[[code format="genbank"]]
 

ALSSTLGEIFLLQNSQAMPDVAWHRLSGEENWVSLLSLHNAQFDLMAKTPYIARHKGTPL

 
[[code]]
=<span style="background-color: transparent; color: #000000; font-family: Arial; font-size: 14px; text-decoration: none; vertical-align: baseline;">LQQIDTALVLQRDAQGQTLPLSPQTKLLFLGGHDTNIANIAGMLGANWQLPQQPDNTPPG</span>= 
[[code format="genbank"]]
 

GGLVFELWQNPDNHQRYVAVKMFYQTMDQLRNAEKLDMKNNPAKIVPITIEGCENEGDNK

 
[[code]]
=<span style="background-color: transparent; color: #000000; font-family: Arial; font-size: 14px; text-decoration: none; vertical-align: baseline;">LCQLETFQKKVAQVIEPACHI</span>= 
[[code format="genbank"]]
 
Joey
ORGANISM: GENE of INTEREST:
NCBI GENE # or RefSeq#: EC# of Enzyme:
CDS GENE SEQUENCE:
AMINO ACID SEQUENCE:
Krishna
ORGANISM :Salmonella
GENE of INTEREST: Acid phosphatase (PDB #2A96) (non essentiality)
NCBI GENE # or RefSeq#: 1255845
EC# of Enzyme: 3.1.3.2
CDS GENE SEQUENCE:
ATGAAAAGTCGTTATTTAGTATTTTTTCTACCACTGATCGTAGCTAAATATACATCAGCAGAAACAGTGCAACCCTTTCATTCTCCTGAAGAAT
CAGTGAACAGTCAGTTCTACTTACCACCACCGCCAGGTAATGATGATCCGGCTTACCGCTATGATAAGGAGGCTTATTTTAAGGGCTATGCGA
TAAAGGGTTCCCCGCGATGGAAACAAGCTGCTGAGGATGCAGATGTAAGCGTGGAAAATATAGCCAGAATATTCTCGCCAGTAGTGGGTGCT
AAAATTAACCCCAAAGATACGCCAGAAACCTGGAATATGTTAAAGAATCTTCTGACAATGGGCGGCTACTACGCTACTGCTTCGGCAAAAAA
ATATTATATGCGTACCCGCCCCTTTGTCTTATTTAATCATTCCACCTGCCGTCCTGAAGATGAGAATACTTTGCGAAAAAATGGCTCTTACCCTT
CCGGGCATACTGCTTATGGTACACTTCTGGCATTAGTATTATCCGAGGCCAGACCGGAACGCGCGCAGGAGCTCGCCAGACGCGGATGGGAG
TTCGGGCAAAGCAGAGTGATATGCGGTGCTCACTGGCAAAGCGATGTTGATGCTGGCCGTTATGTGGGAGCAGTAGAGTTTGCAAGACTGCA
AACAATCCCGGCTTTTCAGAAGTCACTGGCAAAAGTCCGTGAGGAGCTGAACGACAAAAATAATTTATTGAGTAAAGAAGATCACCCCAAAC
TTAATTACTGA
AMINO ACID SEQUENCE:
>gi|16767569|ref|NP_463184.1| non-specific acid phosphatase [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]
MKSRYLVFFLPLIVAKYTSAETVQPFHSPEESVNSQFYLPPPPGNDDPAYRYDKEAYFKGYAIKGSPRWKQAAEDADVSVENIARIFSPVVGAKIN
PKDTPETWNMLKNLLTMGGYYATASAKKYYMRTRPFVLFNHSTCRPEDENTLRKNGSYPSGHTAYGTLLALVLSEARPERAQELARRGWEFGQS
RVICGAHWQSDVDAGRYVGAVEFARLQTIPAFQKSLAKVREELNDKNNLLSKEDHPKLNY
 
[[code]]
**PDB:**http://www.pdb.org/pdb/explore/remediatedSequence.do?structureId=2A96
[[code format="genbank"]]
 
Christina
 
[[code]]
Notes: histidine phosphatase substrate non availability (not in Sigma)
[[code format="genbank"]]
 
PDB: PDB Structure
 
[[code]]
----
**J.C.**
===**Organism:**=== 
Myobacterium tuberculosis
Mycobacterium tuberculosis H37Rv (strain: H37Rv)
**E.C. of Enzyme**:
3.1.3.48
**Gene of Interest:**
====[[#x-JC's research-Organism:-Mycobacterium tuberculosis H37Rv]]Mycobacterium tuberculosis H37Rv==== 
**CDS Gene Sequence**
NCBI Reference Sequence: NC_000962.2
<span class="rprtlinks">GenBank Graphics </span>
>gi|57116681:2507146-2507637 Mycobacterium tuberculosis H37Rv, complete genome GTGTCTGATCCGCTGCACGTCACATTCGTTTGTACGGGCAACATCTGCCGGTCGCCAATGGCCGAGAAGA TGTTCGCCCAACAGCTTCGCCACCGTGGCCTGGGTGACGCGGTGCGAGTGACCAGTGCGGGCACCGGGAA CTGGCATGTAGGCAGTTGCGCCGACGAGCGGGCGGCCGGGGTGTTGCGAGCCCACGGCTACCCTACCGAC CACCGGGCCGCACAAGTCGGCACCGAACACCTGGCGGCAGACCTGTTGGTGGCCTTGGACCGCAACCACG CTCGGCTGTTGCGGCAGCTCGGCGTCGAAGCCGCCCGGGTACGGATGCTGCGGTCATTCGACCCACGCTC GGGAACCCATGCGCTCGATGTCGAGGATCCCTACTATGGCGATCACTCCGACTTCGAGGAGGTCTTCGCC GTCATCGAATCCGCCCTGCCCGGCCTGCACGACTGGGTCGACGAACGTCTCGCGCGGAACGGACCGAGTT GA
**Amino Acid Sequence**
MptpA
>1U2P:A|PDBID|CHAIN|SEQUENCE
MSDPLHVTFVCTGNICRSPMAEKMFAQQLRHRGLGDAVRVTSAGTGNWHVGSCADERAAGVLRAHGYPTDHRAAQVGTEH
LAADLLVALDRNHARLLRQLGVEAARVRMLRSFDPRSGTHALDVEDPYYGDHSDFEEVFAVIESALPGLHDWVDERLARN
GPS
**PDB**
1U2P
[[code format="genbank"]]
 
 
 
Indra
Organism: Shigella flexneri
Gene of Interest: apyrase
NCBI Gene Number: 876677 (periplasmic space,
E.C. Number of Enzyme: 3.6.1.5
CDS Gene Sequence:
 
[[code]]
ATGAAAACCAAAAACTTTCTTCTTTTTTGTATTGCTACAAATATGATTTTTATCCCCTCAGCAAATGCTC TGAAGGCAGAAGGTTTTCTCACTCAACAAACTTCACCAGACAGTTTGTCAATACTTCCGCCGCCTCCGGC AGAGGATTCAGTAGTATTTCTGGCTGACAAAGCTCATTATGAATTCGGCCGCTCGCTCCGGGATGCTAAT CGTGTACGTCTCGCTAGCGAAGATGCATACTACGAGAATTTTGGTCTTGCATTTTCAGATGCTTATGGCA TGGATATTTCAAGGGAAAATACCCCAATCTTATATCAGTTGTTAACACAAGTACTACAGGATAGCCATGA TTACGCCGTGCGTAACGCCAAAGAATATTATAAAAGAGTTCGTCCATTCGTTATTTATAAAGACGCAACC TGTACACCTGATAAAGATGAGAAAATGGCTATCACTGGCTCTTATCCCTCTGGTCATGCATCCTTTGGTT GGGCAGTAGCACTGATACTTGCGGAGATTAATCCTCAACGTAAAGCGGAAATACTTCGACGTGGATATGA GTTTGGAGAAAGTCGGGTCATCTGCGGTGCGCATTGGCAAAGCGATGTAGAGGCTGGGCGTTTAATGGGA GCATCGGTTGTTGCAGTACTTCATAATACACCTGAATTTACCAAAAGCCTTAGCGAAGCCAAAAAAGAGT TTGAAGAATTAAATACTCCTACCAATGAACTGACCCCATAA
[[code format="genbank"]]
 
Amino Acid Sequence:
[[code format="genbank"]]

code
>gi|13448943|ref|NP_085159.1| apyrase [Shigella flexneri 5a] MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPPPPAEDSVVFLADKAHYEFGRSLRDAN RVRLASEDAYYENFGLAFSDAYGMDISRENTPILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDAT CTPDKDEKMAITGSYPSGHASFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMG ASVVAVLHNTPEFTKSLSEAKKEFEELNTPTNELTP
~52% homology to a Escherichia blattae acid phosphatase 1D2T on PDB